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Fig. 2. Relative quantification of gene expression in atrial appendage samples from patients in sinus rhythm (SR) or with atrial fibrillation (AF). (A, C, D) Plot of the mean and individual expression values only for the genes whose expression levels were significantly different (p ≤0.05) between SR (n = 13) and AF (n = 15) in both right and left atrial appendage samples (AA); or between right atrial appendage (RAA) samples from SR patients without atrial dilation (SRnd, n = 8), SR patients with atrial dilation (SRd, n = 8), patients with paroxysmal AF (pAF, n = 8) and patients with persistent-chronic AF (cAF, n = 8) (C); or between left atrial appendage samples (LAA) from SRnd (n = 5) and cAF (n = 7) patients (D). The data are normalized to the validated set of reference genes. (B) Representative immunoblots of EPAC2 and <t>PDE8A</t> in right atrial samples and of PI3Kγ in left atrial samples from patients in SR (n = 8, 6 and 11, respectively) and AF (n = 6, 6 and 11, respectively). The GAPDH levels were used as internal control. Values from SR patients are indicated in black, values from AF patients in red.* indicates p ≤0.05 versus SR. Panels B and C show the genes with chamber-specific differences. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
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Image Search Results


Fig. 2. Relative quantification of gene expression in atrial appendage samples from patients in sinus rhythm (SR) or with atrial fibrillation (AF). (A, C, D) Plot of the mean and individual expression values only for the genes whose expression levels were significantly different (p ≤0.05) between SR (n = 13) and AF (n = 15) in both right and left atrial appendage samples (AA); or between right atrial appendage (RAA) samples from SR patients without atrial dilation (SRnd, n = 8), SR patients with atrial dilation (SRd, n = 8), patients with paroxysmal AF (pAF, n = 8) and patients with persistent-chronic AF (cAF, n = 8) (C); or between left atrial appendage samples (LAA) from SRnd (n = 5) and cAF (n = 7) patients (D). The data are normalized to the validated set of reference genes. (B) Representative immunoblots of EPAC2 and PDE8A in right atrial samples and of PI3Kγ in left atrial samples from patients in SR (n = 8, 6 and 11, respectively) and AF (n = 6, 6 and 11, respectively). The GAPDH levels were used as internal control. Values from SR patients are indicated in black, values from AF patients in red.* indicates p ≤0.05 versus SR. Panels B and C show the genes with chamber-specific differences. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

Journal: Journal of molecular and cellular cardiology

Article Title: Mapping genetic changes in the cAMP-signaling cascade in human atria.

doi: 10.1016/j.yjmcc.2021.02.006

Figure Lengend Snippet: Fig. 2. Relative quantification of gene expression in atrial appendage samples from patients in sinus rhythm (SR) or with atrial fibrillation (AF). (A, C, D) Plot of the mean and individual expression values only for the genes whose expression levels were significantly different (p ≤0.05) between SR (n = 13) and AF (n = 15) in both right and left atrial appendage samples (AA); or between right atrial appendage (RAA) samples from SR patients without atrial dilation (SRnd, n = 8), SR patients with atrial dilation (SRd, n = 8), patients with paroxysmal AF (pAF, n = 8) and patients with persistent-chronic AF (cAF, n = 8) (C); or between left atrial appendage samples (LAA) from SRnd (n = 5) and cAF (n = 7) patients (D). The data are normalized to the validated set of reference genes. (B) Representative immunoblots of EPAC2 and PDE8A in right atrial samples and of PI3Kγ in left atrial samples from patients in SR (n = 8, 6 and 11, respectively) and AF (n = 6, 6 and 11, respectively). The GAPDH levels were used as internal control. Values from SR patients are indicated in black, values from AF patients in red.* indicates p ≤0.05 versus SR. Panels B and C show the genes with chamber-specific differences. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

Article Snippet: For immunoblot analysis, the following antibodies were used: PDE2A (Fabgennix 101AP, rabbit polynclonal antibody, dilution 1:750 in 3% milk, sample cooking 70 ◦C 10-min), PDE8A (Santa Cruz Biotechnology sc-17,232, goat polynclonal antibody, dilution 1:500 in 3% BSA, sample cooking 70 ◦C 10-min), EPAC2 (Cell signaling #4156, mouse monoclonal antibody, dilution 1:250 in 3% BSA, sample cooking 55 ◦C 30-min), PIK3γ (kindly provided by Dr. Emilio Hirsch, mouse polyclonal antibody, dilution 1:100 in 3% BSA, 70 ◦C 10-min), GAPDH (HyTest #5G4 6C5, mouse monoclonal antibody, dilution 1:160,000 in 5% milk).

Techniques: Quantitative Proteomics, Gene Expression, Expressing, Western Blot, Control